This article examines the history of public visitation at the Prof. Mello Leitão Biology Museum (MBML) from 1949 to 2023, including an analysis of the objectives behind scheduled visits in 2022 and 2023. The methodology was based on bibliographic research, document analysis, and thematic categorization of visit purposes. Since its foundation, MBML has prioritized scientific research, but over the years, it has become an important space for education and culture. Its transition to federal management brought significant implications for public engagement. Among the main motivations for guided visits are: Biodiversity and Conservation (30%), History and Culture (26%), and Environmental Education (17%).